Metabolomics services

The main area of expertise of the metabolomics facilities, that are part of the X-omics research infrastructure, consists of state-of-the-art metabolomics, lipidomics and inborn errors of metabolism. The services offered by the facilities are described in more detail below.

Consultancy:

Experimental design
Technical assistance

Sample preparation and storage:

Automated blood sampling and storage

Untargeted metabolomics:

Global deep metabolic profiling (RP or HILIC)
Untargeted exploratory lipodomics

Targeted metabolomics:

Multiple metabolite panels (bile acids, oxylipids, amino acids, acylcarnitines, fatty acids, sterols, etc.)
Quantative lipodomics for >1000 lipid species in 22 lipid classes

Metabolic flux analysis

Data analysis:

Statistical analysis
Metabolite identification

Available equipment for these analyses:

UPLC-High resolution MS (Qtrap 6500, TT6600, QTOF)
GC-MS
NMR
Lipidyzer platform

All services mentioned above are custom services.
Please contact our helpdesk for a quote with turnaround times and prices based on your research question.

Service	Description	Turnaround time	Costs	Equipment	Data Analysis
Consultancy	Discussions on sample collection, experimental design, recommended controls, platform suitability and biochemical coverage	1 - 2 weeks	Free		Not applicable
Blood sample preparation and storage for metabolomics	Automation of the process of blood sampling and storage in order to reduce the need for manual work and monotonous activities and the risk of errors	Several months		Automatic blood collection and transport machines	Not applicable
Quantitative Lipidomics for >1000 lipid species	Flow injection based analysis using the Lipidyzer platform	Custom*	€60 - €200	Lipidyzer platform	Lipidyzer software (integrated)
Bile Acid analysis	LC-MS/MS analysis of >10 bile acids in different matrices	Custom*	€50 - €100	UHPLC-Qtrap 6500	Multiquant
Oxylipids	90 oxylipids in one panel, different matrices	Custom*	€50 - €100	UHPLC-Qtrap 6500	Multiquant
Lipoprotein analysis	Quantification of >175 lipoprotein parameters using the Bruker IVDr platfrom	Custom*	€40 - €100	NMR	Bruker IVDr
Amino Acids	Quantitative analysis of amino acid metabolism	Custom*	€40 - €100	NMR	TopSpin
Central Energy Metabolism	Quantitative analysis of TCA cycel, PPP etc.	Custom*	€40 - €100	NMR	TopSpin
Exploratory Metabolomics	Untargeted Metabolomics	Custom*	€50 - €100	UHPLC-TT6600	MS-DIAL
Exploratory Lipidomics	Untargeted Lipidomics	Custom*	€40 - €100	UHPLC-TT6600	MS-DIAL
Short Chain Fatty Acids	SCFA such as acetic, propionic and butyric acid in different matrices	Custom*	€40 - €100	GC/MS	Excel
Fatty acids	Total FA analysis C2-C24	Custom*	€50 - €100	GC/MS	Excel
Sterols	Distal cholesterol biosynthesis analysis	Custom*	€50 - €100	GC/MS	Excel
Signaling lipid & lipid mediator profiling	Targeted analysis focusing on inflammation, oxidative stress, immune response, incl. a.o. oxylipins, endocannabinoids, bile acids (>200 targets)	2 months	€50 - 100 / sample depending on sample type and study size**	Nominal mass resolution UPLC-MS/MS	In-house pipeline
Global RP deep metabolic profiling	Broad coverage of medium polar metabolites including gut metabolites	2 months	€50 - 100 / sample depending on sample type and study size**	UPLC-HRMS	In-house pipeline
Global HILIC deep metabolic profiling	Broad coverage of polar metabolites including central carbon and energy metabolism	2 months	€50 - 100 / sample depending on sample type and study size**	UPLC-HRMS	In-house pipeline
Lipidomics	Targeted lipid profiling of over 1000 species in 22 lipid classes	2 months	€50 - 100 / sample depending on sample type and study size**	Nominal mass resolution UPLC-MS/MS	In-house pipeline
Custom targeted panels	Targeted analysis with a focus on specific pathways or chemical classes. Examples are biogenic amines, bile acids, acylcarnitines, tryptophan pathway, isotope tracer analysis	Custom*	Custom**	Custom	In-house pipeline
Miniaturized metabolomics analysis	Adapted sample prep of above platforms for sample limited applications such as organoids, organ-on-a-chip, moving towards single cell analysis	Custom*	Custom**	Custom	In-house pipeline
Untargeted metabolomics for heparin plasma or CSF	IEM panel, WES-based analysis, untargeted analysis with/without interpretation	To be agreed upon, for research ± 4 - 6 months	€390-1100	Agilent 6545 QTOF	In-house pipeline

Turnaround time
* Depending on size of sample set.

Costs
** For very large studies (biobanks/clinical trials) contact us for costs.
** Automation and novel HT technologies can change costs.