METABOLOMICS SERVICES

ServiceDescriptionTurnaround timeCostsEquipmentData Analysis
Consultancy Discussions on sample collection, experimental design, recommended controls, platform suitability and biochemical coverage 1 - 2 weeks Free   Not applicable
Blood sample preparation and storage for metabolomics Automation of the process of blood sampling and storage in order to reduce the need for manual work and monotonous activities and the risk of errors Several months   Automatic blood collection and transport machines Not applicable
Quantitative Lipidomics for >1000 lipid species Flow injection based analysis using the Lipidyzer platform Custom* €60 - €200 Lipidyzer platform Lipidyzer software (integrated)
Bile Acid analysis LC-MS/MS analysis of >10 bile acids in different matrices Custom* €50 - €100 UHPLC-Qtrap 6500 Multiquant
Oxylipids 90 oxylipids in one panel, different matrices Custom* €50 - €100 UHPLC-Qtrap 6500 Multiquant
Lipoprotein analysis Quantification of >175 lipoprotein parameters using the Bruker IVDr platfrom Custom* €40 - €100 NMR Bruker IVDr
Amino Acids Quantitative analysis of amino acid metabolism Custom* €40 - €100 NMR TopSpin
Central Energy Metabolism Quantitative analysis of TCA cycel, PPP etc. Custom* €40 - €100 NMR TopSpin
Exploratory Metabolomics Untargeted Metabolomics Custom* €50 - €100 UHPLC-TT6600 MS-DIAL
Exploratory Lipidomics Untargeted Lipidomics Custom* €40 - €100 UHPLC-TT6600 MS-DIAL
Short Chain Fatty Acids SCFA such as acetic, propionic and butyric acid in different matrices Custom* €40 - €100 GC/MS Excel
Fatty acids Total FA analysis C2-C24 Custom* €50 - €100 GC/MS Excel
Sterols Distal cholesterol biosynthesis analysis Custom* €50 - €100 GC/MS Excel
Signaling lipid & lipid mediator profiling Targeted analysis focusing on inflammation, oxidative stress, immune response, incl. a.o. oxylipins, endocannabinoids, bile acids (>200 targets) 2 months €50 - 100 / sample
depending on sample type and study size**
Nominal mass resolution
UPLC-MS/MS
In-house pipeline
Global RP deep metabolic profiling Broad coverage of medium polar metabolites including gut metabolites 2 months €50 - 100 / sample
depending on sample type and study size**
UPLC-HRMS In-house pipeline
Global HILIC deep metabolic profiling Broad coverage of polar metabolites including central carbon and energy metabolism 2 months €50 - 100 / sample
depending on sample type and study size**
UPLC-HRMS In-house pipeline
Lipidomics Targeted lipid profiling of over 1000 species in 22 lipid classes 2 months €50 - 100 / sample
depending on sample type and study size**
Nominal mass resolution
UPLC-MS/MS
In-house pipeline
Custom targeted panels Targeted analysis with a focus on specific pathways or chemical classes. Examples are biogenic amines, bile acids, acylcarnitines, tryptophan pathway, isotope tracer analysis Custom* Custom** Custom In-house pipeline
Miniaturized metabolomics analysis Adapted sample prep of above platforms for sample limited applications such as organoids, organ-on-a-chip, moving towards single cell analysis Custom* Custom** Custom In-house pipeline
Untargeted metabolomics for heparin plasma or CSF IEM panel, WES-based analysis, untargeted analysis with/without interpretation To be agreed upon, for research
± 4 - 6 months
€390-1100 Agilent 6545 QTOF In-house pipeline
Turnaround time
* Depending on size of sample set.

Costs
** For very large studies (biobanks/clinical trials) contact us for costs.
** Automation and novel HT technologies can change costs.

NWO logoThis research was (partially) funded by NWO, project 184.034.019